> - Find a dinosaur from my generation who can suck one into a capillary and > check diffraction at room T. > - Try to find conditions where the crystals don't start to redissolve while > you mount them As a matter of fact, people begin to forget that capillaries are good not only for checking the diffraction, but also for the data collection. However, the laws of Nature do not change with time, and the old dinosaur ways may still work. We just collected at SSRL a room T. data set from crystals that consisted by 75% v/v of water and 25% of a 2500 aa protein complex. The crystals did not like cryoprotectants (including Paratone), but were happy in the capillaries.
On Apr 13, 2012, at 8:32 AM, Phoebe Rice wrote: > I'd suggest: > > - Find a dinosaur from my generation who can suck one into a capillary and > check diffraction at room T. > > - Try using those loops that look like miniature tennis paddles to give the > crystal a little more support > > - To minimize strain on the crystal when pulling it out of the drop, try to > get its long dimension perpendicular to the air-water interface (usually > easier said than done). > > - Try to find conditions where the crystals don't start to redissolve while > you mount them > > ---- Original message ---- >> Date: Fri, 13 Apr 2012 18:51:58 +0800 >> From: CCP4 bulletin board <[email protected]> (on behalf of Prem kumar >> <[email protected]>) >> Subject: [ccp4bb] Crystal behave funny >> To: [email protected] >> >> Hi all, >> I got some Protein + DNA complex crystals (image >> attached) recently. >> They are needle shape some times splitted chromosome >> type crystals. When we pick long needles they bend >> so much than normal crystal but they dont break. The >> small needle dissolve very fast as try to open the >> drop's film. we try to diffract the long needle >> crystals and they diffract up to 20 A resolution. >> Any suggestion how to improve those crystal packing. >> Thanks in advance! >> -Prem >> ________________ >> IMG_1438.JPG (2343k bytes)
