[ccp4bb] minimum protein concentration for NI-NTA column

[Jerry McCully]

Dear All;

       This is a sort of naive question about the NI-NTA affinity purification.

        
       Is the Ni-NTA column from GE health able to capture proteins at 0.2ug/ml 
to 0.5ug/ml?

       IF not, then it is necessary to concentrate the mammalian expression 
supernatant.

      Thanks a lot and best regards,

Jerry McCully