Iodide is a fantastic derivative. One does not need a lot with modern X-ray equipment, careful data collection, and great software.
________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Rajesh Kumar [ccp4...@hotmail.com] Sent: Thursday, May 03, 2012 8:51 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] effective iodide conc. for SAD data Dear All, I have very thin crystals but diffracting. I was not able to handle them easily for iodide soak. I always lost the crystals during manipulation and other big crystals obtained after seeding doesn't even give any diffraction. I tried for co-crystallizing with NaI. The crystals appear only up to 20 mM in 1:2 (3ul drop of 1 ul protein and 2ul reservoir). Is this concentration of iodide is enough for SAD data ( if it had good incorporation) ? I appreciate your help. Thanks Rajesh
