Dear Rajesh, another method to incorporate Iodine into your crystal is by simply placing a drop of KI/I2 solution next to the crystallization drop.
Have a look here: Acta Cryst. (2006). D62, 280-289 New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL) Worked for me with salt concentration sensitive crystal and a derivatization time <10 min. Good luck, Florian Am 03.05.12 15:51, schrieb Rajesh Kumar: > Dear All, > > I have very thin crystals but diffracting. I was not able to handle > them easily for iodide soak. I always lost the crystals during > manipulation and other big crystals obtained after > seeding doesn't even give any diffraction. I tried for > co-crystallizing with NaI. The crystals appear only up to 20 mM in 1:2 > (3ul drop of 1 ul protein and 2ul reservoir). > Is this concentration of iodide is enough for SAD data ( if it had > good incorporation) ? > I appreciate your help. > > Thanks > Rajesh
