In US Fisher Scientific sells CBS scientific , California different ranges of gradient maker. I especially found them useful for my use for density gradient centrifugation. the material used in the manufacture is clear and easy to clean each time. It is kind of not very controlled automatically but works well for membrane preps regularly.
link:http://www.cbsscientific.com/gradientmakers.aspx The tube puncture using a regular syringe works fantastic. On Thu, May 3, 2012 at 11:40 AM, Antony Oliver <antony.oli...@sussex.ac.uk> wrote: > Dear all, > > I find myself working on a number of large multi-protein complexes, and am > likely to need to use Sucrose / Glycerol gradients in preparing them. > IWhilst I can do this manually in the short term, I was wondering if > someone could recommend any manufacturer's (preferably Europe/UK based) that > make suitable systems for: > > Making the gradients in the first place > Fractionating the gradients after they have been in the centrifuge. > > With a great many thanks, > > Tony. > > > --- > Dr Antony W Oliver > > Senior Research Fellow > CR-UK DNA Repair Enzymes Group > Genome Damage and Stability Centre > Science Park Road > University of Sussex > Falmer, Brighton, BN1 9RQ > > email: antony.oli...@sussex.ac.uk > tel (office): +44 (0)1273 678349 > tel (lab): +44 (0)1273 677512 > -- Pius S Padayatti,PhD, Phone: 216-658-4528