Or make it yourself if you got time. Anal Biochem.<http://www.ncbi.nlm.nih.gov/pubmed?term=a%20syringe%20based%20gradient%20former#>1994 Sep;221(2):397-400. A syringe-based gradient former for linear and exponential gradients. Shearer G Jr<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Shearer%20G%20Jr%22%5BAuthor%5D>.
Nian On Thu, May 3, 2012 at 10:40 AM, Antony Oliver <antony.oli...@sussex.ac.uk>wrote: > Dear all, > > I find myself working on a number of large multi-protein complexes, and > am likely to need to use Sucrose / Glycerol gradients in preparing them. > IWhilst I can do this manually in the short term, I was wondering if > someone could recommend any manufacturer's (preferably Europe/UK based) > that make suitable systems for: > > 1. Making the gradients in the first place > 2. Fractionating the gradients after they have been in the centrifuge. > > With a great many thanks, > > Tony. > > > --- > Dr Antony W Oliver > > Senior Research Fellow > CR-UK DNA Repair Enzymes Group > Genome Damage and Stability Centre > Science Park Road > University of Sussex > Falmer, Brighton, BN1 9RQ > > email: antony.oli...@sussex.ac.uk > tel (office): +44 (0)1273 678349 > tel (lab): +44 (0)1273 677512 > >
