Hello Xinghua, I don't think you need to do anything special to ignore overlapping reflections. Mosflm will not integrate them. You biggest concern is that your data will not be complete, which will result in poor density or inability to solve your structure.
Have you looked closely at your images to see if the spots really are too close? You can give some keywords (SEPERATION CLOSE) to mosflm/imosflm in order to encourage very close spots to be properly resolved, but this of course will only work if there are a few pixels of non-reflection between reflections. Scott On May 13, 2012, at 7:22 PM, Xinghua Qin <[email protected]> wrote: > Dear CCP4ers, > > We collected a diffraction dataset with high percentage of spot overlaps, It > would be so kind to tell me how to ignore spot overlap in imosflm and explain > the hazard of high percentage of spot overlaps. > Thanks in advance. > > Best wishes > > Xinghua Qin > -- > Xinghua Qin > State Key Laboratory of Plant Physiology and biochemistry > College of Biological Sciences > China Agricultural University > No.2, Yuan Ming Yuan West Road > Haidian District, Beijing, China 100193 > Tel: +86-10-62732672 > E-mail: [email protected] > >
