Hi Faisal, Please read this thread:
http://www.mail-archive.com/[email protected]/msg00645.html BTW, I had a look at my CCP4(6.2.0) and COOT lib files, it seems the CYS-BME link is not in the mon_lib_list.cif file yet. Instead, I have two MPR-CYS links: MPR-CYS MPR . . CYS . . SS-bridge CYS-MPR CYS . . MPR . . SS-bridge The data of the two look quite the same. Zhijie From: Faisal Tarique Sent: Thursday, May 17, 2012 4:43 AM To: [email protected] Subject: [ccp4bb] Dear All I have one query, i have solved one structure where in which near cys residues i can see density for beta mercaptoethanol (which i have used in my crystallization cocktail ). i have one query when i am taking BME from coot library, i can fit it in density but i do not know how to make disulphide bond ie to connect cysteine with bme. -- Regards Faisal School of Life Sciences JNU
