On Thu, 2012-05-17 at 14:13 +0530, Faisal Tarique wrote: > to make disulphide bond ie to connect cysteine with bme
While I believe that CYS+BME is a correct choice ideologically (you had cysteine and bme reacted with it), note that you can use CME monomer (it's listed as peptide in monomer libraries, so will incorporate fine). On the other hand, I have added BME a number of times in coot and had no problems. Yes, the bond is not showing, likely because coot decides which bonds to draw without much attention to atom type (which is, imho, perfectly fine as fixing this minor non-issue would cost speed). But refmac refines it just fine, and so does rsr in coot. Unless you plan to use coot to make figures, there is no problem. This post may be helpful https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1101&L=CCP4BB&F=&S=&P=118963 Cheers, Ed. -- "I'd jump in myself, if I weren't so good at whistling." Julian, King of Lemurs
