3. Of course if you are working on recombinant protein, you should
double check the sequencing results from the cloning
Preben
On 5/21/12 11:21 PM, Uma Ratu wrote:
Thank you All for you inputs.
Uma
On Mon, May 21, 2012 at 5:06 PM, Van Den Berg, Bert
<[email protected]
<mailto:[email protected]>> wrote:
Yes, as Jacob says, alternative conformation of the serine. Quite
common.
Bert
------------------------------------------------------------------------
*From:* CCP4 bulletin board [[email protected]
<mailto:[email protected]>] on behalf of Uma Ratu
[[email protected] <mailto:[email protected]>]
*Sent:* Monday, May 21, 2012 4:57 PM
*To:* [email protected] <mailto:[email protected]>
*Subject:* [ccp4bb] Serine
Dear All:
Some of serine residues in my model have extra positive Fo-Fc
density at the edge of side chain. Some don't have. It is not like
from phosphates.
I am wondered what is the cause for these extra density. Could
these serines be post-translational modified?
I have the images attached. P289ser-0512-1 does not have the extra
green, where P140ser-0512-1 has.
Thank you for you advice and comment
Uma
--
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Blindern
0318 Oslo, Norway
Email: [email protected]
Tel: +47 2284 0794
http://www.jpmorth.dk
