So if get the gist of the thread right, am I correct in assuming that the last protein structures to be solved strictly by MIR are haemoglobin/myoglobin, lysozyme and chymotrypsin and perhaps one or two more in the late sixties? In which case the answer to the original question about MIR being obsolete, is "yes it is since a long time"?
Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Phil Evans [p...@mrc-lmb.cam.ac.uk] Sent: Wednesday, June 06, 2012 6:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fun Question - Is multiple isomorphous replacement an obsolete technique? No they were not useless! I used them (probably better now with cryo data though) Phil On 6 Jun 2012, at 16:02, Dyda wrote: >> I suspect that pure MIR (without anomalous) was always a fiction. I doubt >> that anyone has ever used it. Heavy atoms always give >> an anomalous signal > >> Phil > > I suspect that there was a time when the anomalous signal in data sets was > fictional. > Before the invent of flash freezing, systematic errors due to decay and the > need > of scaling together many derivative data sets collected on multiple crystals > could render > weak anomalous signal useless. Therefore MIR was needed. Also, current > hardware/software > produces much better reduced data, so weak signals can become useful. > > Fred > > ?[32m******************************************************************************* > Fred Dyda, Ph.D. Phone:301-402-4496 > Laboratory of Molecular Biology Fax: 301-496-0201 > DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov > Bldg. 5. Room 303 > Bethesda, MD 20892-0560 URGENT message e-mail: 2022476...@mms.att.net > Google maps coords: 39.000597, -77.102102 > http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred > *******************************************************************************?[m
