Google yields amongst others: His-tag impact on structure Acta Cryst. (2007). D63, 295301
...quäl dich. -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of weliu Sent: Tuesday, June 26, 2012 6:07 PM To: [email protected] Subject: [ccp4bb] The effect of His-tag location on crystallization Dear all, We crystallized a protein and found that crystal quality greatly depended on the location of His-tag. When a His-tag was added at the C-terminus, only crystalline precipitate or spherical quasi crystals were grown. However, when the His-tag was moved to the N-terminus, single crystals were grown under a number of conditions, and the best one diffracted to 1.7 angstrom after optimization. I was wondering if there were published reports describing similar cases. Thank you in advance Wei Liu
