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Dear Rex,

- - macroseeding
- - microseeding
- - further concentration by addition of precipitant to the reservoir
- - changing crystallisation technique (hanging vs. sitting drops,
crystallisation through membrane, batch crystallisation...)
- - variation of protein concentration
- - add an extra purification step before setting up crystals
- - vary temperature
.....

there is loads, depending on what you mean by 'establised'!

Best
Tim

On 07/04/12 11:33, REX PALMER wrote:
> Does anyone have any new tips/methods for improving crystal size
> once the initial conditions have been established?
> 
> 
> Rex Palmer http://www.bbk.ac.uk/biology/our-staff/emeritus-staff 
> http://rexpalmer2010.homestead.com

- -- 
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

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