Dear Muhammed,
In my experience, crystals like that are likely made up of contaminated or non-homogeneous protein. Have you run NATIVE PAGE and IEF gels to determine the purity of your sample? Is it the correct MW by mass spec without contaminating peaks? Good Luck! Bryan From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Greg Costakes Sent: Tuesday, July 10, 2012 2:18 PM To: [email protected] Subject: Re: [ccp4bb] Crystal Optimization Have you tried multiple rounds of micro-seeding? ------------------------------------------------------------------------------- Greg Costakes PhD Candidate Department of Structural Biology Purdue University Hockmeyer Hall, Room 320 240 S. Martin Jischke Drive, West Lafayette, IN 47907 -------------------------------------------------------------------------------- ** Hard work often pays of in time, but Procrastination always pays off now ** ________________________________ From: "Muhammed bashir Khan" <[email protected]> To: [email protected] Sent: Tuesday, July 10, 2012 1:22:28 PM Subject: [ccp4bb] Crystal Optimization Dear All; Could somebody give a nice suggestion how the following type crystal could be optimized, I almost tried everything. Crystal Image is attached Crystal condition: 20% w/v PEG3350 and 200mM NaCl. Thanks in advance Bashir -- Muhammad Bashir Khan ************************************************** Structural Genome Consortium (SGC). University of Toronto Toronto, Canada -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
