Dear Amr,
Same here. I didn't put DTT or any reducing agent in my buffer. I got
crystals of this protein.
Allan
Quoting [email protected]:
Dear Amr,
Every protein is different, so one cannot make a general remark.
However, in my experience disulfide bonds are normally not affected by
mercaptoethanol or DTT. I have had one protein where the disulfide bonds
were not made, likely because the protein was produced in E.coli.
However, the protein still crystallized.
Good luck!
Herman
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On
Behalf Of amro selem
Sent: Wednesday, July 11, 2012 10:51 AM
To: [email protected]
Subject: [ccp4bb] sulphur bridge
Hallo every one,
i am working on one enzyme who has sulphur bridge , is using 2
mercaptoethnol or DTT during handling this protein for crystallography
is not desirable .
best regards
Amr
--
Allan Pang
PhD Student
G35 Joseph Priestley Building
Queen Mary University of London
London
E1 4NS
Phone number: 02078828480
Twitter: @xerophytes
