Dear Tianlong, You have probably crystallized a contaminant. You can check to see if your unit cell has been seen before in the pdb with this service from Robert Esnouf at Srubi,
http://www.strubi.ox.ac.uk/nearest-cell/nearest-cell.cgi or apply to do a "wide search" whole pdb molecular replacement with up to 100,000 jobs https://portal.sbgrid.org/d/apps/wsmr/ In the past I have used the WS-MR to save a lot of time, as my hot crystals turned out to be not so hot after all. James -- Dr. James W. Murray David Phillips Research Fellow Division of Molecular Biosciences Imperial College, LONDON Tel: +44 (0)20 759 48895 ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Tianlong Zhang [ztlc...@gmail.com] Sent: Wednesday, July 18, 2012 5:46 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] off-topic:a dataset from a polluted sample Dear all, Recently we obtained a dataset and processed it to 1.9 A in P6 (for a/b/c: 49.6, 49.6, 65.3) which suggested there were about 100 amino acids in per AU. Our protein is about 50kDa for a protein complex and expressed in Hi5 cell. Therefore, we dissolved several crystals for N-terminal sequencing and the sequence is totally different from the complex. We suggested that this data set was for a protein from Hi5 cell. Furthermore, the signals of the sequencing were a little weak for determining the protein sequence by blast. I'm looking forward for your suggestions. Enclosed pleased find the the sequencing result. Thanks very much! Best regards. ________________________________ Tianlong Zhang
