Dear Tianlong, you may use Arcimboldo using e.g. a long helix (or b-hairpin) as starting model for de novo phasing. If you succeed to phase your structure, you should be able see if it was part of your protein.
Best wishes Kornelius On Wed, Jul 18, 2012 at 6:46 AM, Tianlong Zhang <[email protected]> wrote: > ** > Dear all, > > Recently we obtained a dataset and processed it to 1.9 A in P6 (for a/b/c: > 49.6, 49.6, 65.3) which suggested there were about 100 amino acids in per > AU. Our protein is about 50kDa for a protein complex and expressed in Hi5 > cell. Therefore, we dissolved several crystals for N-terminal sequencing > and the sequence is totally different from the complex. We suggested that > this data set was for a protein from Hi5 cell. Furthermore, the signals of > the sequencing were a little weak for determining the protein sequence by > blast. I'm looking forward for your suggestions. > > Enclosed pleased find the the sequencing result. > > Thanks very much! > Best regards. > > ------------------------------ > Tianlong Zhang > -- *Kornelius Zeth* *Unidad de Biofisica (CSIC-UPV/EHU) Barrio Sarriena s/n 48940, Leioa, Vizcaya* *SPAIN*
