Dear CCP4ers, We have crystallized a kinase without adding any nuceotide, and the structure does not show any eletron density at the ATP binding site. Yet most of the bacterial-purified proteins of this family and also the structures contains ADP . So we boiled the bacterial-purified protein, and tested the ADP concentration with NADH-coupled ATPase Assay, Surprisingly, the result shows that there is one molecule of ADP per protein. So why would that happen? any suggestions are appreciated Best wishes Ni shi
-- Ni shi State Key Laboratory of Plant Phy siology and biochemistry College of Biological Sciences China Agricultural University No.2, Yuan Ming Yuan West Road Haidian District, Beijing, China 100193 Tel: +86-10-62732672 E-mail: [email protected]
