Dear Ni Shi,
Did you test a crystal, or uncrystallized protein? It may be that due to
crystal packing effects, only protein without ADP crystallizes.
Best regards,
Herman
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On
Behalf Of Ni shi
Sent: Friday, July 27, 2012 5:17 AM
To: [email protected]
Subject: [ccp4bb] ADP binds to the protein in solution but not
show up in the structure
Dear CCP4ers,
We have crystallized a kinase without adding any nuceotide, and
the structure does not show any eletron density at the ATP binding site.
Yet most of the bacterial-purified proteins of this family and also
the structures contains ADP . So we boiled the bacterial-purified
protein, and tested the ADP concentration with NADH-coupled ATPase
Assay, Surprisingly, the result shows that there is one molecule of ADP
per protein. So why would that happen? any suggestions are appreciated
Best wishes
Ni shi
--
Ni shi
State Key Laboratory of Plant Phy
siology and biochemistry
College of Biological Sciences
China Agricultural University
No.2, Yuan Ming Yuan West Road
Haidian District, Beijing, China 100193
Tel: +86-10-62732672
E-mail: [email protected] <mailto:[email protected]>
<mailto:[email protected]>
