A high school intern of mine made one to show the next year's interns.  We have 
not posted to youtube, but perhaps we should.   You also could make one 
yourself in about 5 minutes. :)

We have posted a youtube video on how to use cryotongs: 
http://www.youtube.com/watch?v=ikiF2qpCRKs

Jim

________________________________
From: CCP4 bulletin board [[email protected]] on behalf of Bret Church 
[[email protected]]
Sent: Friday, August 03, 2012 11:18 PM
To: [email protected]
Subject: [ccp4bb] Crystallisation video

Hi,

Does anybody know of a video of setting up crystallisation of HEWL or other 
protein straightforward to crystallise (ie setting up "manually")?  Such a 
setup has the potential even to be popular in schools, I think.  I mean I want 
the experiment shown, and not just a lecture on protein crystallisation.

My searches pick up time lapse video of crystals growing, and robotics setups, 
but I'm talking about setting up crystallisation manually.  Perhaps there are 
dozens of examples out there, but I'm just not getting to them.

I have picked up an article, "Protein Crystallization for X-ray 
Crystallography" at JoVE, from Moshe Dessau and Yorgo Modis  at Yale.  You will 
need a subscription.
http://www.jove.com/video/2285/protein-crystallization-for-x-ray-crystallography

The formal reference is Dessau, M. A., Modis, Y. Protein Crystallization for 
X-ray Crystallography. J. Vis. Exp. (47), e2285, DOI: 10.3791/2285 (2011). 
(www.ncbi.nlm.nih.gov/pubmed/21304455).

The "Visualization" provided by Dessau and Modis is very good, and I recommend 
it.  JoVE seems to have, and be sustaining, high production values…

…but maybe there are other worthwhile video efforts out there which are more 
accessible (ie free).


Cheers,

Bret Church

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