[ccp4bb] best strategy to establish stable expression in mammalian cells

[Jerry McCully]

Dear All;

       We are planning to establish stable CHO or 293 cell clones to express 
some receptors.

        I heard that it is time consuming to select high expression clones 
using pcDNA3.1 vectors. 

      As our crystallographer are dealing with all kinds of proteins, can 
anyone recommend a best strategy for mammalian cell expression?

       Thank you so much and have a nice weekend,

Jerry McCully