Hi Jerry, Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful.
Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html -john On Aug 17, 2012, at 3:38 PM, Jerry McCully wrote: > Dear All; > > We are planning to establish stable CHO or 293 cell clones to express > some receptors. > > I heard that it is time consuming to select high expression clones > using pcDNA3.1 vectors. > > As our crystallographer are dealing with all kinds of proteins, can > anyone recommend a best strategy for mammalian cell expression? > > Thank you so much and have a nice weekend, > > Jerry McCully >
