Dear All, Recently I collected one data set for the protein having SG P222 with a= 36.8, b= 44.7, c= 78.4(reported with compound). I crystallized the protein with same kind of other compound. The diffraction was up to 2.3A. But I am facing problem during indexing. I tried with SGP222 (as reported) but the cell dimension was showing a= 41.5, b= 96.3, c= 112.7 and distortion index around 9.83% whereas for SGP2 its showing a= 96.3, b= 41.5, c= 112.7 with distortion index 0.03%. All spots are taken nicely (Denzo). But with P222 predicted spots are completely different than the real one. So I processed it in P2. Surprisingly when I put the reported SGP222 and cell dimensions during scaling (scalepack), for the same data integrated in P2 (just for curiosity), it is showing better data statistics than P2. Anybody experienced this situation? Am I doing any mistake during indexing? Asking for the suggestions.
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