You may want to check that the distance, 2-theta and wavelength are correct. Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston
________________________________ From: "[email protected]" <[email protected]> To: [email protected] Sent: Tue, September 11, 2012 1:29:26 AM Subject: Re: [ccp4bb] Same protein showing different SG Dear Dipankar, To me, it looks like you have a (slight) problem with your goniometer/detektor in that e.g. the rotation axis is not exactly horizontal/vertical/perpendicular to the beam, or that the detector parameters (especially when using a swing-out) were not completely correctly refined and that this error was fudged in Denzo by having one of the cell-angles not exactly 90°. This could explain while the indexing in P222 did not go well, but the scaling did. If the scaling in P222 went well, this means that the true space group is P222 and that you have to have a critical look at your detector parameters. Best, Herman ________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Dipankar Manna >Sent: Tuesday, September 11, 2012 7:37 AM >To: [email protected] >Subject: [ccp4bb] Same protein showing different SG > > >Dear All, > >Recently I collected one data set for the protein having SG P222 with a= 36.8, >b= 44.7, c= 78.4(reported with compound). I crystallized the protein with same >kind of other compound. The diffraction was up to 2.3A. But I am facing >problem >during indexing. I tried with SGP222 (as reported) but the cell dimension was >showing a= 41.5, b= 96.3, c= 112.7 and distortion index around 9.83% whereas >for >SGP2 its showing a= 96.3, b= 41.5, c= 112.7 with distortion index 0.03%. All >spots are taken nicely (Denzo). But with P222 predicted spots are completely >different than the real one. So I processed it in P2. Surprisingly when I put >the reported SGP222 and cell dimensions during scaling (scalepack), for the >same >data integrated in P2 (just for curiosity), it is showing better data >statistics >than P2. Anybody experienced this situation? Am I doing any mistake during >indexing? Asking for the suggestions. > >Thanks in advance. > >Regards, >Dipankar > > > >Aurigene Discovery Technologies Limited, >#39-40 KIADB Industrial Area, Electronic City, Phase II, >Hosur Road, Bangalore- 560 100, India >Cell: +91-9538631469 | Office Ph : +91 80-66204422 (Extn: 398) | Email ID: >[email protected] > >________________________________ >This e-mail and any files transmitted with it are for the sole use of the >intended recipient(s) and may contain confidential and privileged >information.If >you are not the intended recipient, please contact the sender by reply e-mail >and destroy all copies of the original message.Any unauthorized review, use, >disclosure, dissemination, forwarding,printing or copying of this email or any >action taken in reliance on this e-mail is strictly prohibited and may be >unlawful. > >Visit us at http://www.aurigene.com >
