Dear CCP4 members, Recently, I got a ligand soaking structure to clearly show a large domain (~100 amino acids) movement compared to the no soaking structure. Although there are some reported examples of this enzyme to suggest the flexibility of this large domain which is relevant to substrate binding. *But it is the first time I can see it happen in crystal soaking procedure.* In this case, I am pleased by this result.
My question is, do you have any other example like mine, where domain (or loop) movement is observed *in crystal* during ligand *soaking* procedure? It would be very helpful for me to relate my result to other similar examples. Thank you very much. King regards, Wenhe
