Dear Wenhe, We too have had similar experiences with a couple of other other kinases - CDK2 (PMID: 21291269) and Aurora A (PMID: 22248356), where both soaking and co-crystallization has resulted in large conformational rearrangement.
Best wishes, Mat. ________________________________ From: WENHE ZHONG <[email protected]> To: [email protected] Sent: Tuesday, 9 October 2012, 9:33 Subject: [ccp4bb] Structure example request for large domain movement "in crystallo" soaking Dear CCP4 members, Recently, I got a ligand soaking structure to clearly show a large domain (~100 amino acids) movement compared to the no soaking structure. Although there are some reported examples of this enzyme to suggest the flexibility of this large domain which is relevant to substrate binding. But it is the first time I can see it happen in crystal soaking procedure. In this case, I am pleased by this result. My question is, do you have any other example like mine, where domain (or loop) movement is observed in crystal during ligand soaking procedure? It would be very helpful for me to relate my result to other similar examples. Thank you very much. King regards, Wenhe
