Dear all i have solved a structure ( at 2A resolution) whose Rwork and Rfree is 22 and 25 respectively..the Ramachandran plot shows 90% of the residues in the most favorable region and with 6 residues in generously allowed and no residues in disallowed region. But in some areas i can see density missing for side chains ( in loop regions )..i have question do i need to mutate them to alanine or leave them as such..The density fit analysis in COOT ( traffic light) showing those regions with side chain as red..
thanx in advance Regards Faisal School of Life Sciences JNU
