I would try your motherl liquor augmented with 10 per cent PEG 400 hoping for the best. Better yet mount your crystal inside a capillary like old times & measure room temp data. At least you have something for sure. Finally I have heard (never did it) that if you do not have air on top of your dewar (like nitrogen atmosphere) you do not need cryoprotectant.
In my hands PEG4000 is not a cryoprotectant but adding 5 to 10 per cent PEG400 does the job. If I had an option I would go for room temp data in a home source. Sent from my iPhone On Nov 30, 2012, at 8:22 PM, Yuri Pompeu <[email protected]> wrote: > Dear community, > I have what seems to be a pretty decent single crystal that grew from a > screen set up 2 weeks ago. > I am trying to reproduce it but so far I have not succeeded. I am however > afraid the crystal that did form will start to deteriorate. So this brings me > to dilemma, I feel like I should try and mount this crystal and shoot it. But > since I only have 1 sample, I do not want to mess this up... I am inclined > to try cryo conditions, but I am afraid the addition of a cryo such as > glycerol could destroy the little guy. > The crystal formed in 30% PEG 4000, 0.1M NaCitrate pH5.6 and 0.2M NH4AcO, I > wonder if this is a cryo condition already? > Any suggestions would be appreciated. > > best,
