Yuri Whatever happens, keep the remains of the crystal to make a seed-stock after data collection.
You can also try making a seed stock from the rest of the drop, even if you can't see crystals, and even if it has dried up. Use part of your seed-stock to seed into one or more *random screens* You can make the seed stock by suspending crushed crystals in the reservoir solution that gave the original hit (preferably taken from the original plate as it may have dried out a bi9t). Patrick Allan D’Arcy, Frederic Villarda, May Marsh. 'An automated microseed matrix-screening method for protein crystallization'. Acta Crystallographica section D63 (2007), 550–554. http://scripts.iucr.org/cgi-bin/paper?S0907444907007652 On 1 December 2012 09:22, Yuri Pompeu <yuri.pom...@ufl.edu> wrote: > Dear community, > I have what seems to be a pretty decent single crystal that grew from a > screen set up 2 weeks ago. > I am trying to reproduce it but so far I have not succeeded. I am however > afraid the crystal that did form will start to deteriorate. So this brings > me to dilemma, I feel like I should try and mount this crystal and shoot > it. But since I only have 1 sample, I do not want to mess this up... I am > inclined to try cryo conditions, but I am afraid the addition of a cryo > such as glycerol could destroy the little guy. > The crystal formed in 30% PEG 4000, 0.1M NaCitrate pH5.6 and 0.2M NH4AcO, > I wonder if this is a cryo condition already? > Any suggestions would be appreciated. > > best, > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
