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Dave, You can try any or all of these proteins commercially available and all conditions for crystallization and freezing established. Lysozyme Ferritin Glucose isomerase Myoglobin Proteinase K Thaumatin Trypsin You can follow this link. We used some of the conditions mentioned in their successfully. http://www.rigaku.com/products/protein/recipes Disclaimer: I do not have any commercial interest with Rigaku. Anthony ----------------------------------------------------- Dr. Anthony Addlagatta Center for Chemical Biology Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad AP-500 607, INDIA Tel:91-40-27191812 Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1 ---------- Original Message ----------- From: Harry Powell <ha...@mrc-lmb.cam.ac.uk> To: CCP4BB@JISCMAIL.AC.UK Sent: Mon, 4 Feb 2013 16:10:54 +0000 Subject: Re: [ccp4bb] need some suggestions for crystallization > *********************** > This message has been scanned by the InterScan for CSC SSM at IICT and found > to be free of known security risks. > *********************** > > Hi David > > try going back to the one that started it all,* myoglobin, a recipe > is at > > http://www.rigaku.com/products/protein/recipes > > (* feel free to argue about this) > > On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: > > > So, I know I say this every time I post on this board, but here it > > goes again. > > > > I'm at an undergrad only school, and every 2 years I teach a class > > in protein crystallography. This year I'm being super ambitious, > > and I'm going to take a class of 16 to the synchrotron for data > > collection. It's just an 8 hour thing, to show them the entire > > process. I'm hoping that we can collect 5-6 good data sets while > > there. > > > > I would like them to grow their own crystals, and go collect data. > > Then we'd come back and actually do a molecular replacement (pretty > > easy/standard really). Just to get a feel for how it works. > > > > The protein I do research on is not one that I would push on this, > > as the crystals are hard to grow, they are very soft, and the data > > just isn't the best (resolution issues). I do have a few that will > > work on my proteins, but I was thinking of having others in the > > class grow up classic proteins for data collection. Obviously > > lysozyme is one, but I was wondering what other standard > > bulletproof conditions are out there. > > > > Can you all suggest some protein crystallization conditions (along > > with cryo conditions) for some commercially available proteins? > > I'm looking to get 6-8 different ones (and we'll just take them and > > see how it goes). I wouldn't mind knowing unit cell parameters as > > well (just a citation works, I can have them figure it out). I > > have about 7 weeks to get everything grown and frozen and ready to go. > > > > Any help would be greatly appreciated. It always amazes me how > > helpful this group is. Thank you very much. > > > > Dave > > Harry > -- > Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, > Hills Road, Cambridge, CB2 0QH > Chairman of European Crystallographic Association SIG9 > (Crystallographic Computing) ------- End of Original Message ------- This Mail Scanned by ClamAV and Spammassassin
