-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Dave,
many methods articles mention a small set of commonly used proteins. E.g. Mueller et al, "Optimal fine phi-slicing for single-photon-counting pixel detectors", Acta Cryst D68, p42-56 list Insulin, Lysozyme, Thaumatin, and Thermolysin; Nanao et al, "Improving radiation-damage substructures for RIP", Acta D61, 1227-1237, list Elastase, Insulin, Lysozyme, Ribonuclease A, Thaumatin, and Trypsin. Elastase, though, decided not to crystallise any longer about ten'ish years ago. Attached is a different condition for Lysozyme which I received from Prof. Susana Andrade and which I usually use for teaching. - the crystals grow during lunch time. They can be picked directly and you can show the cryo-protecting effect of Ethylene glycol with increasing concentration. Best, Tim On 02/04/2013 05:03 PM, David Roberts wrote: > So, I know I say this every time I post on this board, but here it > goes again. > > I'm at an undergrad only school, and every 2 years I teach a class > in protein crystallography. This year I'm being super ambitious, > and I'm going to take a class of 16 to the synchrotron for data > collection. It's just an 8 hour thing, to show them the entire > process. I'm hoping that we can collect 5-6 good data sets while > there. > > I would like them to grow their own crystals, and go collect data. > Then we'd come back and actually do a molecular replacement (pretty > easy/standard really). Just to get a feel for how it works. > > The protein I do research on is not one that I would push on this, > as the crystals are hard to grow, they are very soft, and the data > just isn't the best (resolution issues). I do have a few that > will work on my proteins, but I was thinking of having others in > the class grow up classic proteins for data collection. Obviously > lysozyme is one, but I was wondering what other standard > bulletproof conditions are out there. > > Can you all suggest some protein crystallization conditions (along > with cryo conditions) for some commercially available proteins? I'm > looking to get 6-8 different ones (and we'll just take them and see > how it goes). I wouldn't mind knowing unit cell parameters as well > (just a citation works, I can have them figure it out). I have > about 7 weeks to get everything grown and frozen and ready to go. > > Any help would be greatly appreciated. It always amazes me how > helpful this group is. Thank you very much. > > Dave > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRD+M2UxlJ7aRr7hoRAnDhAJ0bE6xl0XDxV6qoKslThPyyVd3CwACgpjnA sIjr3296ejuwby0ZyXLw5po= =JgwT -----END PGP SIGNATURE-----
xtalization_lysozyme.ods
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