If SPG buffer is what I think it is, that means you have a significant concentration of inorganic phosphate, which forms salt crystals when mixed with divalent metal ions.
-Nat On Thu, Feb 7, 2013 at 2:24 PM, amro selem <[email protected]> wrote: > > > > > Hallo my colleagues. > i hope every one doing ok . i did screening since two weeks . i noticed > today this crystals. i don`t know either it salt or protein crystal . my > protein has zero tryptophan so i could distinguish by UV camera. > the condition was conditions: > 0.1M SPG buffer pH 8 and 25%PEG 1500. in addition to Nickle chlorid 1mM. > > > best regards > Amr > > > > > >
