Dear Uma, A very complehensive survey of the state-of-the-art in this topic can be found in the following literature resources:
(1) Garman EF. Radiation damage in macromolecular crystallography: what is it and why should we care? Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51. (2) The January 2013 issue of the Journal of Synchrotron Radiation featuring a special section with papers presented at last year's Intl workshop on X-ray damage of biological crystalline samples. The annotated editorial by Garman and Weik in that issue is also very helpful. Best regards Savvas On 13 Feb 2013, at 23:38, Uma Ratu wrote: > Dear All: > > I plan to use X-ray crystallography method to study the S-nitrosylated > protein structure. > > The native protein crystals diffracted to 2A with synchrontron. I now have > the crystals of S-ntrosylated protein. > > Since S-NO moiety appears to be unstable to synchrotron radiation, could you > advice / comments on the stratage on the data collection of S-nitrosylated > protein crystals? > > The protein crystals did not diffract well with in house X-ray. > > Thank you for your comments. > > Uma >
