Hi, We had a massive phage infection at the SGC in 2004; all our washing and sterilization efforts could only solve the problem temporarily. I then recovered a phage-resistant subclone of BL21(DE3), and prepared derivative strains with pRARE2 (the tRNA plasmid in Rosetta2) or other plasmids. We have expressed thousands of protein since and never had a recurrence of phage problems. Although the resistance is to T1 phages, it seems these are the most common lab infections.
Write me if you want the strains. Opher
