Among many possible reasons, the streaking could be caused mechanical stress to the thin plates during mounting. Have you tried those loops that look like miniature tennis rackets?
Phoebe ________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Rakesh Chatterjee [[email protected]] Sent: Monday, April 01, 2013 10:01 AM To: [email protected] Subject: [ccp4bb] plate crystal optimization hello everyone, i have a protein which co-purifies with a ligand (small molecule ~ 810 Dalton). i didn't know what kind of ligand is it so i am planing to identify it. meanwhile my protein crystallized as plates and while diffracting i found that the crystal is showing a good diffraction pattern upto 2.6 Angstroms in home source Cu K-alpha. however when the crystal rotates between 90 degrees to 135 degrees, the spots become streaky. should i try different cryo-MPD/ PEG400 etc? to circuvent the problem i did some additive screen but was not of much help. any valuable suggestion willbe handfull. thanx in advance rakesh
