-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Afshan,
- - Can you reproduce the crystals in the Linbro plates with the identical solution used for the screening plate? - - Did you compare the composition of the Linbro plate vs. the screening plate? - - Did you check e.g. the pH of the original solution? - - Did you refresh the beta-ME / try a new protein prep? It goes off after some time, as far as I know. Regards, Tim On 04/16/2013 11:21 AM, Afshan Begum wrote: > > > Dear All, > > I have encountered one problem to optimization the crystallization > condition manually. Actually i got the good shape and even size > crystal in a screening plate. The condition are : > > 20% PEG 6000, 0.1M MES pH 6.0 whereas protein in 10mM Na-acetate pH > 5.7 contain 50mM NaCl, 1mM EDTA &5mM BME. When I tried to optimize > this on Linbro the drops are become heavily precipitate even a > mints. I tried so many things to avoid this ppt such as start PEG > conc from 7%, dilute protein at half conc., add more salt in a > reservoir solution but no succeeds. The protein prep is same which > was using to get the Hits. > > > I would be very thankful if you people give me nice suggestions. > > Thanks > > > Best Regards > > AFSHAN - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRbSmWUxlJ7aRr7hoRAstnAKDlOMp84i3k4TxbzyPiXrdrmQmCUgCfWrLs ySGYCLJmu6yEJ7i94AEjqKQ= =EJjS -----END PGP SIGNATURE-----
