Hi Afshan, You mention:
The protein prep is same which was using to get the Hits. So how old is your prep ? Was it stored at 4C or in LN2? Do you know if your protein is stable under the conditions you stored it ? How long after purification did it take you to get those crystals ? How old is your screen ? MES goes bad as well over time. Jürgen ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://lupo.jhsph.edu On Apr 16, 2013, at 5:21, "Afshan Begum" <afshan...@yahoo.com> wrote: > The protein prep is same which was using to get the Hits.
