I would try 2.5M or 3M sodium malonate pH 7 Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email [email protected] ________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Roger Rowlett [[email protected]] Sent: 23 May 2013 21:21 To: [email protected] Subject: Re: [ccp4bb] cryo condition
30% glycerol or 25% glucose should be sufficient for 1-2 M ammonium sulfate. Roger Rowlett On May 23, 2013 5:42 AM, "Faisal Tarique" <[email protected]<mailto:[email protected]>> wrote: Dear all Can anybody tell me the appropriate cryo condition for the crystals obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% glycerol to it but still the ice ring is forming.. Thanx in advance -- Regards Faisal School of Life Sciences JNU
