Just to follow up, the paper that was attached is based on the far more original work by Garman and Mitchell
Elspeth Garman and Edward Mitchell. (1996) Glycerol concentrations required for cryoprotection of 50 typical protein crystallisation solutions. J.Appl. Cryst. 29, 584-587. And there is also a further paper Glycerol concentrations required for the successful vitrification of cocktail conditions in a high-throughput crystallization screen.Kempkes R, Stofko E, Lam K, Snell EH.(2003) Acta Cryst D 64, 287-301. There are a few other papers on high salt concentrations as a cryoprotectant including: Todd Holyoak, Timothy D. Fenn, Mark A. Wilson, Aaron G. Moulin, Dagmar Ringe and Gregory A. Petsko. (2003) Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. Acta D. 59, 2356-2358. Cheers, Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: [email protected] Telepathy: 42.2 GHz Heisenberg was probably here! -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Thibaut Crepin Sent: Thursday, May 23, 2013 6:24 AM To: [email protected] Subject: Re: [ccp4bb] cryo condition Dear Faisal, this paper can be really useful. Regards Thibaut On 23/05/2013 11:42, Faisal Tarique wrote: > > Dear all > > Can anybody tell me the appropriate cryo condition for the crystals > obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% > glycerol to it but still the ice ring is forming.. > > Thanx in advance > -- > Regards > > Faisal > School of Life Sciences > JNU
