It turns out that these images contain two distinct lattices, separated by a rotation of about 5 degrees. Using the development version of iMosflm the two lattices can be easily indexed and integrated. However, the data is quite incomplete due to severe radiation damage.
Andrew On 2 Jul 2013, at 15:43, RHYS GRINTER <[email protected]> wrote: > Hi All, > > I collected some data on the weekend on forked crystal, I collected data on > this crystal at the base before the crystal split into two. > The crystal didn't stand up well to the radiation damage so I shot a number > of places along the crystal and got maybe 45 degrees of good data per > position. Auto-processing failed on all but one data set, this dataset > processed to 3.99 A, but only with around 80% completeness. However looking > at the diffraction images I see spots in the first 45 degrees to at least 3.2 > angstroms. > > I tried quickly to manually process in mosflm, but I noticed that many of the > spots appear to be in fact made up to two very closely located spots. This > data was collected at a micro-focus station so it was impossible to tell this > without careful analysis of the spots. I guess these spots are an indication > that the lattice was splitting even at this point. > > As a relative novice at data processing, I'm wondering if this kind of data > is processable and if so what is the best strategy (or if I should just get > back to the bench and grow some more crystals) and program to use? > > Cheers, > > Rhys
