Hi All, Thanks for all your responses and help. I'll be trying all of these things over the coming couple of weeks.
Rhys ________________________________________ From: [email protected] [[email protected]] On Behalf Of Patrick Shaw Stewart [[email protected]] Sent: 04 July 2013 16:09 To: RHYS GRINTER Subject: Re: [ccp4bb] Split Crystal Dataprocessing Dear Rhys I'm not sure if your group up there in Gla.ac.uk<http://Gla.ac.uk> uses "random" microseeding on a routine basis as soon as you get your first hits, but if you don't I would strongly suggest that you try it. The chances are that you can avoid the sort of problems you are facing. I've posted lots of messages to this bb on this topic, and you can find info by searching in google for MMS or rMMS crystallization. It's very important not to get sucked down one particular path by focussing too much on one hit from a screen. That's why it so helpful to run an rMMS screen as soon as you can. The method also gives you better hits right out of the screen, and also allows you to control the number of crystals per drop. All this is explained at eg http://www.douglas.co.uk/mms.htm Best wishes Patrick On 2 July 2013 15:43, RHYS GRINTER <[email protected]<mailto:[email protected]>> wrote: Hi All, I collected some data on the weekend on forked crystal, I collected data on this crystal at the base before the crystal split into two. The crystal didn't stand up well to the radiation damage so I shot a number of places along the crystal and got maybe 45 degrees of good data per position. Auto-processing failed on all but one data set, this dataset processed to 3.99 A, but only with around 80% completeness. However looking at the diffraction images I see spots in the first 45 degrees to at least 3.2 angstroms. I tried quickly to manually process in mosflm, but I noticed that many of the spots appear to be in fact made up to two very closely located spots. This data was collected at a micro-focus station so it was impossible to tell this without careful analysis of the spots. I guess these spots are an indication that the lattice was splitting even at this point. As a relative novice at data processing, I'm wondering if this kind of data is processable and if so what is the best strategy (or if I should just get back to the bench and grow some more crystals) and program to use? Cheers, Rhys -- [email protected]<mailto:[email protected]> Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
