Hi Mahesh, TESTGEN START 1 END 120 ANGLE 0.5 moving the detector further back or using 2theta and collecting more frames.
When you started shooting at your image you should have checked for overlaps ahead of time. I assume this to about 1.8 Å - maybe it would have been wiser to collect a 2.5 Å dataset. Your corners have zero spots when you crank up the contrast, so you are not using all the available detector area, sure the completeness goes down but you might have avoided overlaps like this. BUT not all is lost, if you dare going to XDS you might be able to squeeze sufficient information out of this data set. Good luck, Jürgen On Aug 16, 2013, at 10:38 AM, Mahesh Lingaraju wrote: Hi CCP4 folks I have a data set which is looks twinned ( see the image-1 - I zoomed on to the image so that one can spot the twinning. Furthermore, the spots are very smeary from ~ 30 - 120 degrees of data collection, see image 2) I tried using HKL2000 and mosflm to process this data but i cannot process it. I was wondering if anyone has any ideas as to how to process this data or comments on whether this data is even useful. Also, I would really appreciate if someone could share their experiences on solving twinning issues during crystal growth Thanks in advance ! Mahesh<image 1.png><image 2.png> ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
