Hi Mahesh In addition to what Mark and Juergen have written, it looks to me like you have very high mosaicity in one direction - the first image shows no distinct lunes; I suspect that may have something to do with the failure to process - but what do you mean when you say you "cannot process it"? Was the failure at the indexing or integration stage? If the failure is at the indexing stage, it's possible that you may make some progress in indexing if you increase the I/sig(I) threshold in Mosflm processing.
Looking closely at some of the spots in the first image it does look like you have more than one lattice there, though I'm not sure I'd describe it as twinning. On 16 Aug 2013, at 15:38, Mahesh Lingaraju wrote: > Hi CCP4 folks > > I have a data set which is looks twinned ( see the image-1 - I zoomed on to > the image so that one can spot the twinning. Furthermore, the spots are very > smeary from ~ 30 - 120 degrees of data collection, see image 2) I tried using > HKL2000 and mosflm to process this data but i cannot process it. I was > wondering if anyone has any ideas as to how to process this data or comments > on whether this data is even useful. Also, I would really appreciate if > someone could share their experiences on solving twinning issues during > crystal growth > > Thanks in advance ! > > Mahesh<image 1.png><image 2.png> Harry -- ** note change of address ** Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
