Hi Bernhard, this one works for us: Sigma BioUltra imidazole, >99.5% by GC, 56749
Cheers, Jan -- Jan Abendroth Emerald BioStructures Seattle / Bainbridge Island WA, USA home: Jan.Abendroth_at_gmail.com work: JAbendroth_at_embios.com http://www.emeraldbiostructures.com On Aug 21, 2013, at 7:33 AM, Bernhard Rupp <hofkristall...@gmail.com> wrote: > Hi Fellows, > > could someone please point me towards the source of a known high purity > imidazole > with low absorbance at 280 nm? I am facing the problem of detecting a low > absorption protein > in high imidazole background after IMAC gradient elution. In the UV spectra > of the > 2 imidazoles I checked there is some contaminant that absorbs at 280… > > Thx, BR > > ---------------------------------------------------------------------------------------- > Bernhard Rupp > Marie Curie Incoming International Fellow > Innsbruck Medical University > Schöpfstrasse 41 > A 6020 Innsbruck – Austria > +43 (676) 571-0536 > bernhard.r...@i-med.ac.at > ---------------------------------------------------------------------------------------- > Dept. of Forensic Crystallography > k.-k. Hofkristallamt > Vista, CA 92084 > 001 (925) 209-7429 > b...@ruppweb.org > b...@hofkristallamt.org > http://www.ruppweb.org/ > ----------------------------------------------------------------------- > >
