Hi Phoebe, Could you please explain me how do you stain the piece of paper? Thank you Armando
El 21/08/2013, a las 17:03, Phoebe A. Rice escribió: > Hi Bernhard, > If your cheap imidazole works fine aside from the absorption problem, > there's always my favorite stone-age detection method: pencil a numbered grid > onto a piece of filter paper, spot the fractions on it, and stain with > coomassie. It'll tell you which fractions to load on a gel, and it goes easy > on the budget as well. > Phoebe > > ++++++++++++++++++++++++++++++++++++++++++ > > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > > 773 834 1723; pr...@uchicago.edu > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > http://www.rsc.org/shop/books/2008/9780854042722.asp > > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bernhard Rupp > [hofkristall...@gmail.com] > Sent: Wednesday, August 21, 2013 9:33 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Low 280 absorbance imidazole? > > Hi Fellows, > > could someone please point me towards the source of a known high purity > imidazole > with low absorbance at 280 nm? I am facing the problem of detecting a low > absorption protein > in high imidazole background after IMAC gradient elution. In the UV spectra > of the > 2 imidazoles I checked there is some contaminant that absorbs at 280… > > Thx, BR > > ---------------------------------------------------------------------------------------- > Bernhard Rupp > Marie Curie Incoming International Fellow > Innsbruck Medical University > Schöpfstrasse 41 > A 6020 Innsbruck – Austria > +43 (676) 571-0536 > bernhard.r...@i-med.ac.at > ---------------------------------------------------------------------------------------- > Dept. of Forensic Crystallography > k.-k. Hofkristallamt > Vista, CA 92084 > 001 (925) 209-7429 > b...@ruppweb.org > b...@hofkristallamt.org > http://www.ruppweb.org/ > ----------------------------------------------------------------------- > >
