Dear all I'm trying to solve a beautiful large crystal that, unfortunately, doesn't go further than 5 A resolution. I believe that in this case, the lack of resolution is due the high solvent content (about 66%). Therefore, my next strategy should be the dehydratation. Yet, I never (sucessfully) did that. I read different approachs, were people equilibrate crystals in dehydratation solution for days, or do more than 20 steps, or add solvents. Since i never had sucess in my trials, I was thinking that someone can suggest a protocol (should I remove all salt?, should I keep the additive concentration?, how much precipitant should I add? how many steps?).
crystal condition: 23% PEG 3350, 0.2M NaCl, 0.1M Tris pH 8.5, 3% galactose (orthorhombic crystals, with about 0.6 x 0.6 mm) all the best, Andre Godoy
