Re: [ccp4bb] crystals with large solvent content -dehydratation

[Leonid Sazanov]

Hi, you could try dehydration in microdialysis buttons - this allows for slow 
gradual increase in PEG over few days and full control of other parameters, 
including lowering salt concentration.
It was the only dehydration method that worked well for our large membrane 
protein:
http://www.ncbi.nlm.nih.gov/pubmed/21822288
Described in more detail here:
http://www.ncbi.nlm.nih.gov/pubmed/24059518