Dear all, please note:
*AnoXtal: a new platform dedicated to protein crystallization under
anaerobic conditions.*
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The Metalloproteins Group of the Institut de Biologie Structurale will
receive demands for anaerobic protein crystallization, from both the
academic and private communities, starting January 2014. Anaerobic
crystallization does not only concern proteins that contain
air-sensitive metal clusters and active sites but it also applies to
proteins which bind organic cofactors, such as FAD, or have thiol groups
that can get oxidized to disulfide bridges. The available set-up
consists of a Gryphon robot and a CrysCam visualization motorized camera
(/ArtRobins/) installed inside a customized glove-box (/Jacomex/). The
typical oxygen concentration in the box is 3-5 ppm. Thirteen
crystallization kits with a total of 1248 conditions will be available.
Each of the 96 wells of the crystallization plate can hold three protein
drops so that the maximum total number of drops with different
compositions can be 3744. Drops will be monitored periodically, up to
six weeks, and the corresponding images will be sent to the user.If
usable crystals are obtained, the user may (i) decide to reproduce them
locally or (ii) have them mounted, flash cooled and sent to the chosen
destination.
The platform AnoXtal is ISO9001 certified and will be available for
six-week periods, alternated with equivalent periods of unavailability.
The calendar and further information are now available at the IBS web
site:
http://www.ibs.fr/platforms/other-facilities-developments/crystallisation-under-anaerobic/(link
to be changed soon).
Contacts for the AnoXtal platform : [email protected]
<mailto:[email protected]>and [email protected]
<mailto:[email protected]>
