Hi all; I have crystal of membrane protein (110 kDa) in several different conditions. I optimized several of them and tested it for their diffraction. It does not goes behind 10A at CLS and SSRL.
The protein is purified by two steps nickel and gel filtration. The crystal grow very fast and reach to maximum size of 500*70*70 in approximately 30hrs. I mostly tried with different temperatures as well as additives. I am trying with Micro Batch crystallization in order to slow down the crystal growth. Any new idea which I can try will be highly appreciated from your own experience.... Thanks... -- Muhammad Bashir Khan ************************************************** Department of Biochemistry University of Alberta Edmonton Canada
