Junyu,
I haven't tried it personally with this particular solution, but I have
found that 30% glucose can pretty much cryoprotect any condition I have
tried it with. If necessary, add cryoprotectant solution (mother liquor
+ 30% glucose) gradually to minimize osmotic shock and potential
cracking of crystals.
You can try this without crystals to see if the solution vitrifies as a
clear solid in liquid nitrogen. If it freezes clear, it is very likely
to work fine with your crystals.
Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [email protected]
On 12/16/2013 4:36 PM, Xiao, Junyu wrote:
Dear all, sorry if this topic does not interest you. I wonder whether
anyone has experience with freezing crystals grown in ~0.2 M Magnesium
Formate. Garman and Mitchell suggested that "A major anomaly is
solution 44, 0.2 M magnesium formate, which requires 50% glycerol for
cryoprotection" in their 1996 paper (J Appl. Cryst. 29, 584-587).
Since 50% glycerol is kind of harsh, I wonder whether anyone has
tried alternative cryo protectant. Your kind help will be highly
appreciated.
Best regards,
Junyu
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Junyu Xiao, Ph.D.
University of California, San Diego
Leichtag Room 283
9500 Gilman Drive, 0721
La Jolla, CA 92093-0721
Lab phone: 858-822-0684
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